热休克蛋白增加运动发酵单胞菌乙醇产量的研究

Adaptation Mediated by Hsp can Improve the Resistance to Adversity and Ethanol Production of Zymomonas mobilis

目的:对含有Hsp基因的代谢工程运动发酵单胞菌进行热适应处理,用于提高其乙醇产量和对高糖的耐受性。方法:用含有Hsp基因的多顺反子质粒转化运动发酵单胞菌,经过多天37℃热适应后,在玉米水解液中测定该多顺反子对运动发酵单胞菌发酵产生的影响。结果:在37℃发酵条件下,在糖浓度为28%的玉米水解液中,与对照组相比,经过热适应的含Hsp基因的代谢工程菌的乙醇产量显著提高。结论:Hsp基因能够显著提高运动发酵单胞菌的乙醇产量。

Objective： Hsp was used to mediated the adversity adaptation of Zymomonas mobilis to increase it＇s resistance to the adversity and ethanol production. Method： Zymomonas mobilis was transformed by plasmid containing Hsp gene, then incubated at 37℃ for adversity adaptation. After days of treatment, the performance of ethanol production of the adapted strain was analyzed in corn hydrolysis media. Result： After adaptation, the production of ethanol by the Zymomonas mobilis with Hsp was 1.55 times more than it＇s wild type counterpart in corn hydrolysis with sugar concentration as 28% at 37℃.Conclusion： Hsp gene can significantly increase ethanol production of Zymomonas mobilis at high temperature and high sugar concentration.