上皮性卵巢肿瘤组织中膜型基质金属蛋白酶-1(MT1-MMP)的表达及临床意义

Expression of Membrane Type-1 Metalloproteinase in Tissue of Epithelial Tumor of Ovary and Its Clinical Significance

目的:研究膜型基质金属蛋白酶-1(MT1-MMP)在上皮性卵巢肿瘤组织中的表达及临床意义。方法:应用免疫组化EnVision法检测40例上皮性卵巢癌(A组)、20例交界性上皮性卵巢肿瘤(B组)、20例良性上皮性卵巢肿瘤(C组)和20例正常卵巢组织(D组)中MT1-MMP的表达和预后。结果:MT1-MMP在上皮性卵巢癌中的阳性率(95%)显著高于良性卵巢肿瘤(45%)和正常卵巢(35%),A组中的强阳性率(77.5%)显著高于B组(30%)、C组(10%)和D组(10%)(P<0.05);D组中未绝经者的阳性率(66.7%)显著高于绝经者(9.1%)(P<0.05);晚期上皮性卵巢癌中的强阳性率(100%)显著高于早期的强阳性率(52.6%)(P<0.05);中低分化(G2～G3级)上皮性卵巢癌中的强阳性率(86.7%)显著高于高分化(G1级)的强阳性率(50%)(P<0.05);有淋巴转移的上皮性卵巢癌中的强阳性率(94.4%)显著高于无淋巴转移组的强阳性率(63.6%)(P<0.05)。在单因素生存分析中,A组中MT1-MMP的表达与患者预后不良有关;B组中MT1-MMP表达强阳性组(++～+++)的复发率(66.7%)显著高于MT1-MMP表达阴性～弱阳性组(-～+)的复发率(7.1%)(P<0.05)。结论:MT1-MMP可能参与卵巢的正常生理功能,它的异常高表达在上皮性卵巢癌的浸润、转移和交界性上皮性卵巢肿瘤的复发中起了重要作用,MT1-MMP的表达与患者预后不良有关。

Objective： To stUdy the expression of membrane type-1 metalloproteinase （MT1-MMP） in tissue of epithelial tumor of ovary, and to discuss its clinical significance. Methods： Immunohistochemical staining （EnVision method） was used to detect the expression of MT1-MMP in 40 specimens of epithelial ovarian carcinomas （group A）, 20 epithelial borderline ovarian tumors （group B）, 20 epithelial benign ovarian tumors （group C） and 20 normal ovarian tissues （group D）. Results： The positivity of MT1-MMP in group A （95%） was statistically higher than that in group C （45%） and group D （35%） （P〈0.05）, and furthermore its strong positivity in epithelial ovarian carcinomas （77.5%） was stasistically higher than that in epithelial borderline ovarian tumors（30%）, in group B （10%） and in group D （10%） （P〈0.05）. In group D, the positivity of MT1-MMP in non-menopause subgroup （66.7%） was statistically higher than that in menopause subgroup （9.1%）（P〈0.05）. The strong positivity of MT1-MMP in advanced epithelial ovarian carcinomas （100%） was statistically higher than that in earlier stage （52.6%）（P〈0.05）. The strong positivity of MT1-MMP in moderately-poor-differentiated epithelial ovarian carcinomas（86.7%）was statistically higher than that in well-differentiated group （50%）（P〈0.05）. In epithelial ovarian carcinomas with lymphatic metastasis, the strong positivity of MT1-MMP （94.4%） was statistically higher than that without lymphatic metastasis （63.6%）（P〈0.05）. In single-factor analysis of survival, the overexpression of MT1-MMP in group A indicated a poor prognosis. For group B, recurrence rate of strong positive expression group （＋＋ to ＋＋＋）（66.7%） was statistically higher than that of negative-weak-positive expression group （- to ＋） （7.1%）（P〈0.05）. Conclusion： MT1-MMP may participate in normal ovarian function. Overexpression of MT1-MMP indicates that it plays an important part in invasion and metastasis of epithelial ovarian carcinomas and in recurrence of epithelial borderline ovarian tumors. The overexpression of MT1-MMP indicates a poor prognosis.