猪前体脂肪细胞的培养方法及生物学特征研究

Research on the Porcine Preadipocyte Culture and Its Biological Characteristics

[目的]研究大白猪前体脂肪细胞培养方法。[方法]选用出生3 d的仔猪脂肪组织,采用原代消化细胞法进行分离,培养后,研究猪脂肪组织增生的生物学特征。[结果]结果表明,培养的原代细胞经传代后成分均一、生长旺盛充脂率高,培养细胞含有可被油红O染色的脂滴,具有前脂肪细胞的典型特征。[结论]试验建立的培养方法适用于脂肪细胞的培养和纯化,脂肪细胞特征典型。

[Objective] The method of porcine preadipoeyte culture was researched. [Method] The adipose tissue from 3 day - age pig was cultured after isolated with the primary cell digestion method and then the biological characteristics of pig adipose tissue reproduction was researched. [ Results] The resuits showed that the primary cells cultured were highly homogeneous, vigour growth and high differentiatian rate. The cultured cells could be stained with oil red O,with iypical character of preadipocyte. [Conclusion] The experimental culture method was suitable for the cell culture and purification and there were the typical characteristics of preadipoeyte.