地尔硫抑制酶修饰低密度脂蛋白对外周血中树突状细胞分化成熟的影响研究

Diltiazem Inhibit Differention and Maturation of Dendritic Cells in Peripheral Blood by Enzymatically Modified LDL

目的:探讨酶修饰低密度脂蛋白(E-LDL)对外周血中树突状细胞(DC)分化成熟的影响以及地尔硫对其的影响作用。方法:梯度离心法分离人外周血单核细胞,用含重组人粒细胞-巨噬细胞集落刺激因子和重组人白介素4的Cellgro培养液培养5d,使其分化为DC。分别用E-LDL、地尔硫加E-LDL刺激DC48h后,镜下观察DC的形态,采用流式细胞术检测DC表型(CD1a、CD80、CD86、HLA-DR)的表达,并设磷酸缓冲盐对照组进行比较。结果:光镜下DC形态无明显变化,各组DC的CD1a、CD80、CD86和HLA-DR的表达有显著性差异(P<0.05),E-LDL处理后DC表面抗原的表达较对照组升高,地尔硫加E-LDL处理后DC表面抗原的表达较E-LDL组下调。结论:E-LDL可促进DC的分化成熟,地尔硫对E-LDL激活DC具有抑制作用。

OBJECTIVE： To investigate the effect of enzymatically modified low density lipoprotein （E - LDL） on differation and maturation of human dendritic cells（DC） in peripheral blood and the effect of diltiazem on it. METHODS： Human monoeytes were isolated by using gradient centrifugation method and cultured in DC Cellgro medium containing rhGM CSF and rhIL - 4 for 5 days, then DC was then obtained. DC were treated with E - LDL or E - LDL plus diltiazem for 48 hours,the morphological changes were observed under the microscope, and the FACS was used to investigate the expression of DC （CDla, CD80, CD86 and HLA - DR） .PBS group was set up for comparison. RESULTS： The morphological changes were nor observed. The expressions of CDla, CD80, CD86 and HLA DR in each groups were significant different（P〈0.05） . The immunophenotypic expression of DC in E - LDL group increased compared with the control group. The expression reduced significantly in diltiazem plus E- LDL group compared with E- LDL group. CONCLUSIONS： It suggests that E- LDL can promote the differation and maturation of DC which may be inhibited by diltiazem.