土壤放线菌Streptomyces sp.2215代谢物的分离鉴定及抗肿瘤活性研究

Purification and Identification of Antitumor Secondary Metabolites from Soil Streptomyces sp.2215

土壤来源的放线菌Streptomyces sp.2215经发酵罐发酵,培养液经乙酸乙酯提取,硅胶柱层析、C18反相色谱、Sephadex LH-20、及TLC和HPLC分离纯化得到四个化合物,并通过理化性质,IR,FAB-MS,1H,13C NMR,1H-1HCOSY,HSQC,HMBC等鉴定为单亚砜棘霉素(monosulfoxide quinomycine,1)、棘霉素(echinomycin,2)、喹喔啉-2-羧酸(3)、十六烷酸(4)。其中化合物1、2经MTT法测定体外对K562细胞具有抑制活性,其IC50值分别为34.0、1.5 ng/mL。

Streptomyces sp. 2215 was fermented in fermentation tank, and the broth was extracted with EtOAe and purified by silica chromatography, C18 chromatography, Sephadex LH-20, preparative TLC and HPLC. Four compounds were isolated and identified to be monosulfoxide quinomycine （ 1 ）, echinomycin （ quinomycine A,2） ,2-quinoxalinecarboxylic acid （3）, palmitic acid （4） by IR, FAB-MS,^1H,^13C NMR,^1H -^1H COSY, HSQC, HMBC etc. Compounds 1 and 2 showed inhibitory activity on the growth of K562 cells with IC50 34.0 and 1.5 ng/mL respectively by MTT assay in vitro.