摘要
土壤来源的放线菌Streptomyces sp.2215经发酵罐发酵,培养液经乙酸乙酯提取,硅胶柱层析、C18反相色谱、Sephadex LH-20、及TLC和HPLC分离纯化得到四个化合物,并通过理化性质,IR,FAB-MS,1H,13C NMR,1H-1HCOSY,HSQC,HMBC等鉴定为单亚砜棘霉素(monosulfoxide quinomycine,1)、棘霉素(echinomycin,2)、喹喔啉-2-羧酸(3)、十六烷酸(4)。其中化合物1、2经MTT法测定体外对K562细胞具有抑制活性,其IC50值分别为34.0、1.5 ng/mL。
Streptomyces sp. 2215 was fermented in fermentation tank, and the broth was extracted with EtOAe and purified by silica chromatography, C18 chromatography, Sephadex LH-20, preparative TLC and HPLC. Four compounds were isolated and identified to be monosulfoxide quinomycine ( 1 ), echinomycin ( quinomycine A,2) ,2-quinoxalinecarboxylic acid (3), palmitic acid (4) by IR, FAB-MS,^1H,^13C NMR,^1H -^1H COSY, HSQC, HMBC etc. Compounds 1 and 2 showed inhibitory activity on the growth of K562 cells with IC50 34.0 and 1.5 ng/mL respectively by MTT assay in vitro.
出处
《天然产物研究与开发》
CAS
CSCD
2009年第2期235-238,共4页
Natural Product Research and Development
基金
国家自然科学基金(30472082)