若羌大枣多糖的分离纯化及抗氧化活性的研究

Purification and Antioxidant Activity of Polysaccharide from Zizyphus jujuba Mill in Xinjiang

对新疆若羌大枣多糖的分离纯化和抗氧化活性作了研究。结果表明:将大枣干粉经热水浸提、乙醇沉淀,得到的大枣多糖粗品经索氏提取法脱脂、Sevag法除蛋白,双氧水脱色,透析法除单糖,用DEAE-纤维素层析,经过蒸馏水、0～1 mol/L NaCl可得到大枣多糖的3个级分,JPSN、JPSA1、JPSA2;然后用SephadexG-100柱进一步鉴定3个级分的纯度,经鉴定JPSN、JPSA1均为单一组分,而JPSA2得到JPSA2B1、JPSA2B21、JPSA2B33个亚组分。通过红外光谱扫描,呈现多糖类吸收峰。若羌大枣粗多糖对DPPH、超氧阴离子、羟基自由基、NO2-都有较好的清除作用。

This pape studied on the purification and antioxidant properties of polysaceharide from Zizyphus jujuba Mill. Crude polysaccharide was obtained from Zizyphus jujuba Mill using following methods：extracting polysaccharide with hot water,precipitating with ethyl alcohol. Free proteins and pigment were removed by Sevage method and H2O2. Three polysaccharide fractions were gotten by DEAE-Cellulose column with eluent of water,O-1 mol/L NaC1. JPSN,JPSA1 ,JPSA2 was further purified on Sephadex G-100 column, three subfractions of JPSA2 （ JPSA2 B1 , JPSA2 B21, JPSA2 B3 ） were obtained. And JPSN ,JPSA would be studied too. SephadexG-100 column chromatography and pc illustrated that the fraction JPSN,JPSA1 was homogenous polysaccharide. The results demonstrated that polysaccharides have antioxidant properties which may scavenge DPPH, superoxide radicals, hydroxyl radical.