褪黑素对胰腺癌细胞株SW1990体外增殖及凋亡的影响

Effects of melatonin on proliferation and apoptosis in human pancreatic cancer cell line SW1990

目的探讨褪黑素（MT）体外抑制胰腺癌细胞株SW1990增殖及诱导其凋亡的作用。方法以不同浓度的MT（0．1、0．5、1．0、2．5及5．0mmol／L）处理体外培养的胰腺癌细胞株SW1990细胞24、48、72h。用MTr法测定细胞增殖，以AnnexinV／PI检测细胞凋亡，流式细胞仪分析细胞周期及Western blotting检测细胞Bcl-2、Bax蛋白表达。结果MT呈浓度和时间依赖性抑制SW1990细胞的增殖。0．1—5．0mmol／LMT作用48h后，细胞的增殖抑制率为7．4％-85．8％。1．0～5．0mmol／LMT作用48h后，G。／G，期比例为72．6％～85．3％，细胞凋亡率为21．5％-41．7％，同时Bcl-2蛋白表达下调，Bcl-2／Bax比值下降。结论MT可以抑制SW1990细胞增殖，其机制可能与上调Bax表达，下调Bcl-2表达，促进细胞凋亡，将细胞周期阻止于G0／G1期有关。

Objective To investigate the effect of melatonin （MT） on the proliferation and apoptosis in human pancreatic cancer cell SW1990 in vitro. Methods SW1990 cell line were treated with MT at different concentrations （0.1, 0.5, 1.0, 2.5 and 5.0 mmol/L） and at different time points （24 h, 48 h and 72 h）. Cell proliferation was evaluated by MTT and apoptosis was determined by AnnexinV/PI, cell cycle was determined by flow cytometry, and Western Blot was used to detect the expression of Bel-2 and Bax. Results MT could inhibit the proliferation of SW1990 cell in a time and dose dependent manner. 48 h after 0.1 - 5.0 mmoL/L MT treatment, the proliferation inhibitory rate was 7.4% -85.8% , the proportion of SW1990 cell in phase G0/G1 was 72.6% - 85.33%. 48 h after 1 - 5.0 mmol/L MT treatment, the apoptosis rate was 21.5% 41.7%, and the expression of Bcl-2 was down-regulated and the ratio of Bcl-2/Bax decreased. Conclusions MT could inhibit the proliferation of SW1990 pancreatic cancer cells by up-regulating the expression of Bax and down-regulating the expression of Bcl-2, as well as enhancing apoptosis and blocking SW1990 cell in phase G0/G1.