浊度法研究测定氯霉素制剂的效价

Study on determination of Chloramphenicol in its preparation by turbidimetric method

目的采用浊度法研究测定氯霉素其制剂效价的方法。方法以大肠杆菌为试验菌,加菌量1.2%～1.5%(V/V),(37±0.5)℃培养4h左右测定。结果抗生素线性浓度为2.5～11.5μ/ml,一剂量法片剂的平均回收率为100.7%,RSD为1.8%(n=9);滴眼剂的平均回收率为101.3%,RSD为1.7%(n=9)。结论本方法灵敏,快速,影响因素较少,可作为测定氯霉素其制剂效价的方法。

Objective To establish a turbidimetric method for the content determination of Chloramphenicol in its preparation . Methods The test organism was Eschericbia coli and cultured with fresh medium （ pH 7.0）. Adjust spore suspension of Escherichia coli inoculate with a 1.2% - 1.5% （V/V） ratio , at 37℃ ± 0. 5℃ for about 4 h. Results The liner range was 2.5 - 11.5 p, omL. The average recovery of Chloramphenicol tablets was 100.7%, （RSD = 1.8%, n = 9） . The average recovery of Chloramphenicol eye drops was 101.3% , （RSD = 1.7%, n = 9） . Conclusion The method was sensitive , rapid and feasible to determine Chloramphenieol in its preparation.