不同转移潜能小鼠肝癌细胞系趋化因子与受体基因表达谱的分析

Chemokine and chemokine receptor gene expression profiles of mouse hepatocellular carcinoma cell lines with differently metastatic potentials

目的:寻找与小鼠肝癌淋巴道器官特异性转移相关的趋化因子及其受体。方法:应用趋化因子及其受体分类基因芯片技术,分析高、低淋巴道转移潜能小鼠肝癌细胞株HCa-F、HCa-P不同的趋化因子及其受体的表达差异。结果:HCa-F和HCa-P细胞有不同的趋化因子及受体的表达谱。在基因芯片的128个候选基因中,HCa-F与HCa-P相比有19个基因上调、下调>2倍。上调的19个基因中包括G蛋白偶联受体2和CXCL1/2、HIF1和NF-kB等;下调的有19个,包括CC和CCR家族成员和CXC家族成员。结论:不同的趋化因子及其受体表达谱可能与肿瘤的淋巴管转移潜能相关。应用分类基因芯片筛选出的基因为肿瘤转移预测及干预措施可能有重要的意义。

OBJECTIVE： To find the chemokines and chemokine receptors associated with lymphatic organ-specific metastasis in mouse hepatocellular carcinoma. METHODS： Gene expression profiles of Hca-F and Hca-P, two mouse hepatocellular carcinoma cell lines with differently lymphatic metastatic potentials, were investigated by using chemokine and chemokine receptor microar ray. RESULTS： There were different gene expression profiles be tween Hca-F and Hca-P. Of 128 candidate genes, 19 genes, such as GPR2, CXCL1/2, HIF-1 and NF- κB, were upregulated in the Hca-F cell line compared with the Hca-P cell line; 19 genes, such as members of CC, CCR and CXC family, were downregulated in the Hca-F cell line compared with the Hca-P cell line. CONCLUSIONS： The different gene expressions of chemokine and chemokine receptors are related to differently lymphatic metastatic potentials. The screened genes using classified gene chip maybe play an important role in predicting metastasis and targeting intervention.