微波提取高效液相色谱法测定土壤中15种痕量多环芳烃

Determination of Trace Polycyclic Aromatic Hydrocarbons in Environmental Soil by Using Microwave Assisted Extraction Coupled with High Performance Liquid Chromatography

采用微波提取结合高效液相色谱技术测定了土壤中15种PAHs的含量。比较了用微波提取、索氏提取和超声萃取3种土壤样品的前处理方法对多环芳烃测定的影响，考察了色谱柱的性能、梯度洗脱条件的优化、荧光检测波长程序变换及柱温等因素对15种PAHs组分之间分离的影响。经优亿后的HPLC方法对15种PAHs的最低检测限为0．10-0．80μg／kg。相对标准偏差为0．60％～4．60％，方法的回收率为58．1％-97．8％。实验结果表明，该方法具有高效、快速、灵敏等特点，可以用于环境土壤样品中痕量PAHs的检测。

The analytical method for the determination of trace polycyclic aromatic hydrocarbons（PAHs） in environmental soil by using microwave assisted extraction （MAE） coupled with high performance liquid chromatography（HPLC） was described. The recoveries of / obtaining with the different pretreatment method, including microwave assisted extraction（MAE）, Soxhlet extraction（SE）and Ultrasonic extraction（UE） ,for the 15 PAHs were compared and it was seen that the sample pretreatment steps played a key role during the analysis due to the very low PAHs contents. The Chromatographic conditions, such as the choice of C18 column, the gradient elution procedure and fluorescence detection wavelength, were optimized. The detection limits for the 15 PAHs were from 0.10μg/kg to 0.80μg/kg, and the relative standard deviations（RSDs） were from 0.60 % to 4.60 % . The recoveries of this method were from 58.1% to 97.8 %. The results indicated that this method is fast, convenient, sensitive, and suitable for the determination of trace PAHs in environmental soil.