蔗糖和外源激素对马铃薯脱毒试管苗的影响

Effects of Sucrose and Exogenous Hormones on Plantlets in vitro in Potato

本试验以马铃薯品种大西洋脱毒试管苗为材料,采用正交试验设计,研究了蔗糖和外源激素(6-BA、B9、NAA)对脱毒试管苗生长状况的影响。研究结果表明,对于试管苗的不同指标,其最优组合不尽相同。有利于株高的组合:3%蔗糖+0.01mg·L-16-BA+20mg·L-1B9+0.1mg·L-1NAA;有利于茎粗的组合:12%蔗糖+1mg·L-16-BA+20mg·L-1B9+0.02mg·L-1NAA;有利于茎节数的组合:3%蔗糖+0.1mg·L-16-BA+35mg·L-1B9+0.1mg·L-1NAA;有利于叶片数的组合:3%蔗糖+0.1mg·L-16-BA+20mg·L-1B9+0.02mg·L-1NAA。

Effects of sucrose and exogenous hormones （6-BA, B9 and NAA） on the growth of plantlets in vitro were studied in an orthogonal design using the cultivar Atlantic as plant materials. For various growth indexes of plantlets in vitro, the best treatment combination was different. For plant height： 3% sucrose ＋ 0.01 mg·L^-1 6-BA ＋ 20mg·L^-1 B9 ＋ 0.1 mg·L^-1 NAA; for stem diameter： 12% sucrose ＋ 1 mg·L^-1 6-BA ＋ 20 mg·L^-1 B9 ＋ 0.02 mg·L^-1 NAA; for node number： 3% sucrose ＋ 0.1 mg·L^-1 6-BA ＋ 35 mg·L^-1 B9 ＋ 0.1 mg·L^-1 NAA; for leaf number： 3% sucrose ＋ 0.1mg·L^-1 6-BA＋20 mg·L^-1 B9 ＋ 0.02 mg·L^-1 NAA.