人参总皂苷对人红白血病细胞株(K562)中STAT5表达的影响

Effect of STAT5 in the Regulation of Total Saponins of Panax Ginseng to K562 Cells

旨在探讨人参总皂苷对K562细胞STAT5表达的影响。MTT法显示TSPG对K562细胞增殖抑制程度呈剂量与时间依赖性增加,且呈正相关关系。流式细胞术表明TSPG能阻止K562细胞从G0/G1期向S、G2/M期移行。激光共聚焦显微镜观察可见,TSPG 200 mg/L作用K562细胞24 h,胞浆中的STAT5荧光强度增加,而胞核内STAT5荧光强度减弱。Westernblotting结果显示,TSPG作用K562细胞6、24、48 h,胞核中STAT5表达较对照组减少,TSPG作用72 h胞核蛋白中STAT5表达增加;TSPG作用K562细胞6、12、24、48、72 h,胞浆内STAT5表达增加。TSPG能减少K562胞核中STAT5的表达,这可能是TSPG抑制K562细胞增殖的作用机制之一。

The research was to investigate the effect of total saponins of panax ginseng on expression of STATS in K.562 cells. The effect of TSPG on proliferation of K562 cells was examined by gTT,flow cytometry. The expression of STAT5 in K562 cells was investigated by laser scanning confocal microscope （LSCM） , and STAT5 in the cytoplasma and nucleus of K.562 cells were determined by Western blotting analysis, respectively. MTT showed that proliferation of K562 cells was inhibited by TSPG in time-dependent and con- centration-dependent manner. Flow cytometry analysis indicated that TSPG blocked K562 cell cycle from G0/G1 to S and G2/M phase. Under LSCM, fluorescence intensity in cytoplasma of K562 cells stimulated by 200 mg/L TSPG for 24 h was stronger than that of control group, while fluorescence intensity in nucleus of K562 cells became weaker. As determined by Western blotting,the expression of STAT5 in nucleus of K562 cells decreased after TSPG treatment for 6 ,24 and 48 h, however, the expression of STATS in nucleus of K562 cell induced by TSPG for 72 h increased compared with control group. The expression of STAT5 in cytoplasm of K562 cells increased after TSPG treatment for 6 , 12,24,48 and 72 h. These results suggested that TSPG reduced the expression of STAT5 in nucleus of K562 cell, which might be based on mechanisms by which TSPG inhibit K562 cells to proliferate.