超临界CO2快速膨胀制备鱼腥草挥发油脂质体

Preparation of Liposome of Volatile Oil from Houttuynia cordata Thunb.by Rapid Expansion of Supercritical Carbon Dioxide

采用改进的超临界CO2快速膨胀方法制备了鱼腥草挥发油脂质体,并对其进行了表征.考察了压力、温度、共溶剂乙醇用量等因素对脂质体制备的影响,在压力30MPa、温度338K及乙醇用量15%条件下,脂质体包封率为83.2%,载药量为5.2%,平均粒径为332.5nm.脂质体包封率、溶出度、稳定性等理化性质均符合中国药典的规定,载药量满足注射剂给药量的要求.

The liposome of volatile oil from Houttuynia cordata Thunb. was prepared by modified rapid expansion of supereritical solutions （RESS）, and physicochemical properties of liposome were characterized. The effects of pressure, temperature of supercritical CO2 and cosolvent （CH3CH2OH） amount on the liposome formation process were examined. Entrapment rate, drug loading, and average size of liposome were obtained as 83.2%, 5.2% and 332.5 nm respectively under the conditions of 30 MPa, 338 K and CH3CH2OH 15%. The results showed that the physicochemical properties including entrapment rate, dissolution rate and stability conformed to the standards of Chinese pharmacopoeia, and drug loading met the needs of injection dosage.