基于Taguchi试验设计稻草发酵产纤维素酶的优化

Optimization of the Fermentation of Straw to Produce Cellulase Based on Taguchi Design

采用Taguchi设计法综合考察接种量、硫酸铵、磷酸二氢钾、七水硫酸镁等因素对实验室筛选出的1株木霉菌种进行产纤维素酶的影响。结果表明,接种量对产酶的影响最大,其次是硫酸铵、七水硫酸镁,而磷酸二氢钾对产酶酶活的影响最小;Taguchi设计优化后得到产纤维素酶的最优条件为:接种量为8%,硫酸铵、磷酸二氢钾、七水硫酸镁添加量分别为0.6%、0.25%和0.03%。在最优条件下预期纤维素酶活能达到6.276μmol/g·min,而验证性试验证明在优化条件下,纤维素酶活达到了5.222μmol/g·min,比优化前的纤维素酶活平均值3.128μmol/g·min增加了2.094μmol/g·min,表明Taguchi方法对于产纤维素酶优化具有较好的应用价值。

Taguchi design method was adopted to investigate the effects of inoculation quantity, （NH4）2SO4, KH2PO4, MgSO4·7H2O on cellulase production by a trichoderma strain （screened in the Lab）. The results showed that the inoculation quantity had the strongest influence and then followed by （NH4）2SO4, MgSO4·7H2O and KH2PO4.The optimum conditions for cellulase production were summed up as folllows by use of Tagnchi Design Method： inoculation quantity was 8 %, the best addition levels of （NH4）2SO4, KH2PO4 and MgSO4·7H2O were 0.6 %, 0.25 % and 0.03 % respectively. Under the most optimized conditions, the activity of the produced cellulase could achieve 6.726 μmol/ （g·min） theoretically. The validation experiments showed that the average activity of the produced cellulase under the optimized conditions was up to 5.222 μmol/ （g·min） （inceased by 2.094μmol/（g·min） than before （3.128 μmol/g·min））, which proved that Taguchi design method was of good application value in the optimization of cellulase production conditions.