小鼠狼疮样肾炎模型的建立

Establishment of a murine lupus nephritis model

目的:研制可诱导的小鼠狼疮样肾炎模型。方法:本研究利用正常成年DBA/2J与C57BL/6杂交子一代(B6D2F1)(雄性)小鼠为受体鼠,接受多次大量DBA/2J(雄性)小鼠脾脏、胸腺、淋巴结细胞的静脉输注(每周2次,共4次),2个月左右大部分受体小鼠出现局部胡须脱落、腹水、毛发变色等现象。结果:ELISA显示血清中IgG、IgG1和IgG2含量明显增加(P<0.01)。考马斯亮蓝染色法检测显示,近80%实验组小鼠出现蛋白尿,而对照组小鼠未发现存在尿蛋白。病理学分析显示,皮肤、肠道、肝脏和肾脏均有炎性细胞浸润,尤以肝脏、肾脏病变为著。结论:提示已初步建立了较稳定的小鼠狼疮样肾炎模型,为相关后续研究提供了有益的资料。

Objective ：To develop an inducible mouse lupus nephritis model. Methods： Male mice （B6D2F1）hybridized from normal mature DBA/2J and C57BL/6 were used as recipients. The spleen, thymus and lymph node cells from male DBA/2J mice were injected intravenously twice a week. After two months, the majority of recipient mice showed whisker fall, ascites and hair color change. Results： The ELISA results showed that the contents of IgG, IgG1 and IgG2 in serum were increased markedly （P 〈0.01 ）. Coomassie brilliant blue staining showed that nearly 80% of the experimental mice had albuminuria, while the control group did not. The pathological analysis showed that the skin, intestine, liver and kidneys of the experimental group had inflammatory cell infiltration, especially the liver and kidneys. Conclusion： The mouse lupus nephritis model is constructed successfully and will benefit the subsequent research.