盐酸戊乙奎醚对大鼠感染性脑水肿AQP4表达的影响

Effects of Penehychdine on the Expression of AQP4 in Endotoxin-induced Brain Edema in Rats

目的:研究盐酸戊乙奎醚对感染性脑水肿保护作用的机制。方法:采用左颈内动脉注射脂多糖复制大鼠感染性脑水肿模型,将84只雄性SD大鼠随机分为对照组(C组)、水肿组(I组)、盐酸戊乙奎醚治疗组(P组)。检测6 h、12 h、24 h、48 h时各组大鼠脑组织含水量、病理形态;脑组织SOD活性、MDA含量;免疫组化法及RT-PCR法检测AQP4蛋白分布与AQP4mRNA含量。结果:(1)与C组相比,I组、P组脑含水量、MDA含量均升高,同时SOD活性、AQP4蛋白、AQP4mRNA表达均减少(P<0.01)。与对应时间点P组相比,I组脑含水量、MDA含量均升高,同时SOD活性、AQP4蛋白、AQP4mRNA表达均减少,于24 h达高峰,48 h仍在较高水平(P<0.01)。(2)脑组织光镜检查:I组大鼠脑组织损伤严重,P组损伤明显减轻。结论:盐酸戊乙奎醚能在一定程度上可减轻感染所致的脑组织损伤,上调AQP4表达,对脑水肿有一定治疗作用,其机制可能与抗氧化作用有关。

Objective： To investigate the effects of penehyclidine on endotoxin-induced brain edema and its possible mechanism. Methods： The infection brain edema model was induced by LPS 150 μg injection in the internal carotid artery. 84 male SD rats were randomly divided into 3 groups （n = 28 ） ：control group （group C ） , edema group （group I） , penehyclidine group （group P）. The animals were decapitated at 6 h,12 h,24 h and 48h after operation （n =7 at each time point in each group）. The brain water content, SOD activity, MDA content is measured, and AQP4 protein is detected by immunohistoehemistry, as well as AQP4mRNA by RT-PCR. Results： 1. Compared with the group C, the brain water content, MDA content was increased （ P 〈 0.01 ） ; SOD activity, AQP4 protein, AQP4mRNA expression reduction at the same time point in group I and group P （ P 〈 0. 01 ） ; compared with the Group P, the brain water content, MDA content was increased （ P 〈 0.01 ） ;SOD activity, AQP4 protein, AQP4mRNA expression reduction at the same time point in group I（ P 〈 0. 01 ）, and reached the peak at 24 h, remained at a relatively high level in 48 h. 2. Light and electron microscopy： the brain injury seriously in group I, the slightly attenuated in group P. Conclusion：Penehyelidine can reduce blood brain barrier injury, increase AQP4 expression, and alleviate brain edema, to a certain extent of the treatment, which may be related to the antioxidant effects.