中药地锦草芹菜素糖苷类化合物

Apigenin glycosides from Euphorbia humifusa Wild.

为了研究中药地锦草抗HBV的活性物质基础,采用大孔树脂、Sephadex LH-20、MCI GEL CHP 20P等色谱方法,从地锦草70%乙醇提取物中分离得到5个芹菜素苷类化合物,通过MS、NMR、2D-NMR等波谱分析手段鉴定了化合物的结构,分别为:芹菜素-7-O-(6"-O-没食子酰)-β-D-葡萄糖苷(1),芹菜素-7-O-β-D-芹糖(1→2)-β-D-葡萄糖苷(2),芹菜素-7-O-β-D-芦丁糖苷(3),芹菜素-7-O-β-D-葡萄糖苷(4),芹菜素(5)。化合物1为新化合物,化合物2、3为首次从该植物中分得。

The investigation on the herbal of Euphorbia humifusa Wild. was carried out in order to find its anti-HBV constituents. The isolation and purification were performed by chromatography such as Sephadex LH-20, MCI GEL CHP 20P, etc. Based on the spectral analysis, five apigenin glycosides were identified as apigenin-7-O-（ 6″-O-galloyl）-β-D-glucopyranoside （1）, apigenin-7-O-β-D-apiofuranosyl （1→2）-β-D-glucopyranoside （2）, apigenin-7-O-β-D-lutinoside （3）, apigenin-7-O-β-D-glucopyranside （4） and apigenin （5）. Among them, compound 1 is a new compound, compound 2 and 3 were isolated from this plant for the first time.