摘要
以生长快、分解乳糖或在乳中生长良好、对常用抗生素普遍敏感且敏感限符合最高允许残留限量(MRL)为原则,对9株标准菌株进行了普通培养基和乳清的生长曲线观察,15种抗生素敏感性试验,并选出5株菌进行了9种抗生素的敏感限测定。结果选出2株嗜热脂肪芽孢杆菌、1株金黄色葡萄球菌和1株嗜热链球菌为开发试剂盒的指示菌。药敏稳定性、培养条件优化及活力稳定性(保质期)尚待进一步研究。
The indicator strains should grow fast, decompose lactose or grow well in milk, be generally sensitive to commonly used antibiotics, and have sensitive restriction in line with the allowable maximum residue limit （MRL）. With these requirments as the selection principle, the growth curves of nine standard strains in common medium and whey were observed. And the sensitivity of them to 15 kinds of antibiotics was also determined. Two strains of Geobacillus stearothermophilus, one strain of Staphylococcus aureus and one strain of Streptococcus thermophilus were selected as indicator strains for development of kit. Stability of drug sensitivity, optimization of culture conditions and stability of activity （shelf life）are needed further study.
出处
《畜牧与饲料科学》
2009年第3期40-42,共3页
Animal Husbandry and Feed Science
关键词
抗生素残留
指示菌
药敏试验
试剂盒
antibiotic residue
indicator strain
drug susceptibility test
test kit
