奶牛布鲁氏菌的PCR鉴定

Identification of Brucella in Dairy Cow by PCR

采集内蒙古某地区的可疑感染布鲁氏菌病奶牛的乳样及奶牛流产胎儿的肺、肝、脾等相关病料,分离并培养出11株病原菌,经BCSP31-PCR、VirB8-PCR、多重PCR及AMOS-PCR4种PCR方法鉴定,证实11株病原菌均为布鲁氏菌,并将11株菌定型为牛种5,6,9或3b型。

The milk samples and relative samples of lung, liver, spleen etc. in aborted fetus of dairy cow infected with Brucella suspiciously in an area of Inner Mongolia were collected and 11 pathogenic strains were isolate and cultured. 11 pathogenic strains were identified as BruceUa and confirmed as B. abortus biovar 5, 6, 9 or 3b by using 4 kinds of PCR methods as BCSP31-PCR, VirB8-PCR, multiplex PCR and AMOS-PCR.