红豆杉离体胚培养快速育苗研究

Studies on the Seedling Culture of Yew Embryo in Vitro

采用Ｂ５和ＭＳ相组合的ＢＭ基本培养基和适宜浓度的激素（２，４－Ｄ，６－ＢＡ和ＧＡ３）能解除红豆杉种胚的休眠．单因子试验和不同组合的正交试验结果显示：单独使用２，４－Ｄ０．１ｍｇ／Ｌ，６－ＢＡ２ｍｇ／Ｌ和ＧＡ３２ｍｇ／Ｌ均在一定程度上促使红豆杉种胚的萌发，萌发率分别为７０％，５５％和２８．６％；最佳正交组合为６－ＢＡ１ｍｇ／Ｌ＋２，４－Ｄ０．１ｍｇ／Ｌ＋ＡＣ４ｇ／Ｌ，萌发率达８１％．

The dormancy of Yew embryo (T. chinesis var meirei ) was broken effectively by means of embryo culture in vitro in the modified medium. Experiments of single factor and orthogonal tests showed that using 2.4 D 0.1mg/L, 6 BA 2mg/L, or GA 3 2mg/L individually could enhance the germination of Yew embryo. The germination rates were 70%, 55%, 28.6% respectively. The best combination of the hormones was 6 BA 1mg/L, 2.4 D 0.1mg/L and AC 4g/L, and the best germination rate was 81%.