不同月龄阿尔茨海默病模型小鼠行为学改变与胆碱酯酶活性的关系

Relationship between changes of behavior and cholinesterase activity in APP transgenic mice

目的:研究不同月龄淀粉样前体蛋白(APP)转基因小鼠学习记忆功能,脑皮质,海马胆碱酯酶以及突触蛋白-Ⅰ的变化.方法:采用Morris水迷宫测试学习、记忆功能,比色法测定胆碱酯酶活性,免疫组织化学法测定突触蛋白-Ⅰ变化.结果:6,9,12mo APP阳性转基因小鼠(APP+)学习、记忆能力明显降低,9～12月龄降低尤为显著;皮层,海马胆碱酯酶活性降低,且皮层胆碱酯酶活性下降更为明显;海马CAⅠ区突触蛋白-Ⅰ的表达随着月龄的增加而减少.结论:APP转基因小鼠学习、记忆功能与海马内胆碱酯酶活性及突触蛋白-Ⅰ的表达呈负相关.说明在阿尔茨海默病(AD)的发病机制中乙酰胆碱的变化和神经突触的萎缩都是重要原因.

AIM： To observe the changes of learning and memory ability, cholinesterase activity and synaptic proteins in hippocampus and cortex in transgcnie mice. METHODS： Morris water maze was used to study the learning and memory ability of action,colorimetry was used to detect the cholinesterase activity and synaptic proteins were observed by histochemistry. RESULTS： APP positive transgenic mice of different ages showed a decline in learning and memory, especially the age of 9 - 12 months. The cholinesterase activity in hippocampus and cortex greatly degraded and the degradation in cholinesterase activity of cortex was more severe. The quantity of synaptophysin- I decreased along with the increasing age in APP positive transgenic mice. CONCLUSION：The learning and memory ability is negatively correlated with synaptic proteins and the cholinesterase activity in APP transgenic mice.