FHIT、HPV16/18在宫颈鳞癌中的表达及意义

The expression and significance of the FHIT and HPV16/18 in cervical cancer

目的探讨脆性组氨酸三联体(Fragile histidine triad)基因(FHIT)及HPV16/18与宫颈癌发病的关系。方法采用免疫组化法检测50例宫颈鳞癌组织、50例正常宫颈组织中的FHIT基因表达情况和HPV感染情况。结果FHIT在正常宫颈组织中的阳性表达率为72%(36/50),在宫颈鳞癌组织中的阳性表达率为32%(16/50),明显低于正常宫颈组织组P<0.001。HPV16/18在正常宫颈组织的阳性率为12%(6/50),在宫颈鳞癌组的阳性率为72%(36/50),两组间比较有统计学差异P<0.001。相关分析提示FHIT蛋白缺失与HPV16/18感染无相关关系。结论FHIT基因缺失及HPV16/18感染与宫颈癌的发生有关。HPV感染与FHIT蛋白缺失异常是导致宫颈鳞癌的两个独立原因。

Objective To evaluate the relationship between fragile histidine triad （FHIT） transcription abnormalities and HPV16/18 infection and human cervical tumorigenesis. Methods The immunohistochemisloy was used to detect the expression of FHIT and HPV16/18 in normal tissue and in cancer tissue. Results In normal cervices and in cervical cancers, the positive rates of FHIT protein expression amounted to 72% （36/50） and 32% （16/50） respectively, and there were statistics significant differences （P〈0. 001）. The positive rates of HPV16/18 were 36% （18/50） and 72% （36/50） respectively, and there were also statistics significant differences between the normal cervices group and cervical cancers group （P〈0. 05）. The analysis show that there were no correlation between the expression of HPV16/18 and FHIT protein （r=-0. 089）. Conclusion The default of FHIT and infection of HPV16/18 are correlated with cervical cancer. HPV infection and FHIT protein default are the two independent causes of cervical cancer.