人胎回肠树突状细胞的形态发生分析

A Morphogenesis Study of Dendritic Cells in Human Fetal Ileum

目的:探索人胎回肠树突状细胞(dendritic cell,DC)的形态发生规律。方法:应用免疫组织化学术和体视学方法对人胎回肠DC的出现时间、形态参数进行分析。结果:第11w时人胎回肠固有层开始出现阳性DC,其数量随胎龄的增大而逐渐增多;回肠固有层及集合淋巴小结(Peyer’s Patches,PP)阳性DC的数密度均随胎龄的增加而增大,而集合淋巴小结DC的数密度比同期固有层的要高(P<0.05);平均表面积随胎龄的增加呈现逐渐减小的趋势,而集合淋巴小结DC的平均表面积比固有层的要小(P<0.001);集合淋巴小结DC的表面积体积比比固有层的要小(P<0.05)。结论:人胎回肠DC的数量随胎龄的增加而增多,固有层DC的形状、大小和数量均与集合淋巴小结DC有差异。

Objective： To investigate the morphagenetic rule of dendritic cells （ DC ） in human fetal ileum. Methods： Using immunohistochemical technique and stereological method, the initiation of appearance and morphometric parameters of DC in human fetal ileum were studied. Results： DC began to appear in the ileum at 11^th week of gestation. The numbers of DC increased with fetal age. The numerical density （Nv） of DC in the lamina propria （LP） and Peyer＇ s patches （PP） increased with fetal age. These parameters of PP DC were higher than those of LP DC at the same stage （P 〈 0.05）. The mean surface area of DC tended to decrease with fetal age. The mean surface area of PP Ds was smaller than that of LP DC in fetal ileum （P 〈 0.001 ）. The ratio of surface to volume of PP DC was less than that of LP DC （ P 〈 0.05 ）. Conclusion： The quantities of DC in human fetal ileum increase with fetal age. The quantity, size and shape of PP DC are different from those of LP DC.