不同糖浓度K-H液对大鼠离体心脏缺血/再灌注损伤的影响

The Influence of Different Glucose Concentrations on Isolated Myocardial Ischemia/Reperfusion Injury

目的:观察不同糖浓度的Krebs-Henseleit(K-H)液对大鼠离体心脏缺血/再灌注损伤的影响。方法:采用Langendorff大鼠离体心脏灌流技术制备心脏缺血/再灌注损伤模型,用不同糖浓度的K-H液灌流心脏,灌流结束后测定心肌组织中肌酸激酶(CK)、乳酸脱氢酶(LDH)、谷胱甘肽-过氧化物酶(GSH-PX)的活性以及一氧化氮(NO)、丙二醛(MDA)的含量。结果:1.5倍糖浓度组(3g.L-1)能显著提高大鼠心肌组织中LDH、CK、GSH-PX的活性(P<0.05),提高NO(P<0.05)、降低MDA(P<0.05)的含量。结论:1.5倍糖浓度组(3g.L-1)对大鼠缺血/再灌注心肌有保护作用,可能是通过增加心肌细胞膜稳定性、增强大鼠抗氧化酶系统功能、松弛血管平滑肌增加血供、抑制心肌缺血/再灌注损伤时的脂质过氧化损伤来实现的。

Objective： To observe the influence of different concentrations of Glucose in Krebs-Henseleit （K-H） liquid on isehemia/repeffusion injury in rats with isolated working hearts. Methods： The heart models of ischemia/reperfusion injury were built with Langendorff isolated perfusion technology. The different concentrations of Glucose in K-H liquid were used for reperfusion. At the end of the reperfusion, the activities of Creatine Kinase（CK）, Lactate Dchydrogenase（ LDH）, Glutathione peroxidase（GSH-Px） and the concentrations of Nitric Oxide（ NO）, Malondialdehyde（MDA） in the myocardial tissue were examined. Results： The group of 1.5 times Glucose concentration showed an increase in the enzymatic activities of LDH, CK, GSH-Px and an increase in the concentration of NO and a decrease in the concentration of MDA in the myocardial tissue. Conclusion： The group of 1.5-times-Glucose-concentration has a protective effect on myocardial ischemia/reperfusion injury because it improves the stability of myocardial cell membrane, enhances anti-oxidative enzyme activity, improves blood supply by relieving blood vessel smooth muscle, restrains the lipid hydroperoxide injury when myocardial tissue suffers from ischemia/reperfusion injury.