摘要
文章以马铃薯克新19号为材料,提取植株叶片总DNA,利用同源序列法,根据GenBank中已登陆番茄的Fe-SODmRNA序列,设计1对引物,经PCR扩增获得了大小为1413bp的片段。序列分析表明,预测其含有4个外显子和3个内含子,编码了145个氨基酸序列,分子质量为16.255ku,等电点为5.88。其编码的氨基酸序列包含了两个Fe-SOD蛋白的保守结构域,与NCBI数据库中其他植物的Fe-SOD蛋白同源性很高,与番茄中58～203相关片段同源性高达93.79%。该基因已在GenBank上注册,登录号为EU545469。
In this experiment, the total DNA was extracted from the variety Kexin No.19, and a pair of primers was designed according to the conserved regions of the Fe-SOD mRNA from tomato in GenBank. A 1 413 bp fragment was got by PCR amplieation. The results of sequence analysis showed that the sequence contained four extrons and three introns, and encoded 145 amino acids. The molecular weight was 16.255 ku, and its isoeleetrie was 5.88. This sequence had two domains encoding Fe-SOD protein. The sequence with Fe-SOD of other plants was blasted on NCBI website, and it was found that they had high homology. The homology was 93.7% compared with the relative part 58-203 in tomato. This gene had been submitted in GenBank, and its access number was EU545469.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2009年第4期17-20,共4页
Journal of Northeast Agricultural University
基金
国家科技支撑计划项目(2006BAD01A06-1-3)
