摘要
系统阐述了玉米品种鉴定技术的产生、发展及研究现状。由于种子科学技术的发展及相关学科的渗透,玉米品种鉴定技术在近期内已由传统的形态学和化学方法发展到了现代的各种电泳技术,包括醇溶蛋白的等电聚焦电泳(zein-IEF)、同工酶电泳(isozymeelectrophoresis)、SDS聚丙烯酰胺凝胶电泳(SDSPAGE)、酸性系统下聚丙烯酰胺凝胶电泳(acidic-PAGE)等。此外,许多学者还进行了分子标记技术(如RFLP与RAPD等)、色谱分析及映像分析等方面的探讨。
This paper systematically reviewed the history and current status of seed identification technology of maize cultivars. Following the rapid development of seed science and the continuing introduction of biochemical means, the technology had been developed from traditionally morphological and chemical procedures to different kinds of modern electrophoretic techniques, such as zein-IEF, isozyme electrophoresis, SDS-PAGE,, acidic PAGE etc. The molecular marker (RFLP and RAPD etc. ), the chromatography, and the image analysis had also been exploited by some researchers.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
1998年第1期68-74,共7页
Journal of China Agricultural University
基金
国家自然科学基金!39470470
关键词
玉米
品种鉴定
电泳
杂交种
自交系
maize
research advance
varietal identification
electrophoresis