COPD患者血浆IL-17、sICAM-1的研究

Study on changes of IL-17 and sICAM-1 in the serum of patients suffering from COPD and its clinical significance with airway inflammation

目的探讨慢性阻塞性肺疾病(COPD)患者血浆白细胞介素-17(IL-17)、可溶性细胞间粘附分子-1(sICAM-1)浓度的变化及相关性。方法30例急性加重期和稳定期COPD患者、25例健康体检者,分别查血常规、测定肺功能,用ELISA法检测血浆IL-17、sICAM-1浓度。结果同一患者COPD急性加重期血浆IL-17浓度、sICAM-1浓度均明显高于稳定期(P<0.01,P<0.01);患者COPD急性加重期血浆IL-17浓度、sICAM-1浓度明显高于健康对照组(P<0.01,P<0.01),患者COPD稳定期血浆IL-17浓度、sICAM-1浓度明显高于健康对照组(P<0.01,P<0.01)且在急性加重期IL-17、sICAM-1与Neu/Leu%呈正相关(r=0.824,P<0.01;r=0.827,P<0.01)。结论COPD急性加重期及稳定期IL-17、sICAM-1水平显著升高,提示IL-17、sICAM-1参与了COPD的发病,可能是引起肺内炎症细胞浸润及肺实质破坏的主要原因之一。

Objective To investigate the clinical significance of changes of serum levels IL-17 and sICAM-1 in patients with chronic obstructive pulmonary disease （COPD）. Methods 30 patients with COPD during acute exacerbation and stable period, and 23 normal persons （control group） were chosen. In testing the pulmonary function, the ratio of eutrophils/leukocytes Neu/Leu%, enzyme linked immunosorbent assay （ELISA） was used to measure IL-17 and sICAM-1 in all. Results The patients＇plasma IL-17 concentration, sICAM-1 concentrations were significantly higher in the acute exacerbation of COPD than the stabilization period （P 〈 0. 01, P 〈 0. 01 ）. Also the measures of plasma IL-17 concentration, sICAM-1 concentration was significantly higher in the acute exacerbation of COPD than the healthy control group （P 〈 0. 01, P 〈 0. 01 ）. The patients＇plasma IL-17 concentration, sICAM-1 concentration were significantly higher in the stable COPD than the control group （P 〈0. 01 ,P 〈0. 01 ）. In the acute exacerbation, IL-17, sICAM-1 and Neu/Leu% was positively correlated （r = 0. 824,P 〈 0. 01 ;r = 0. 827,P 〈 0. 01 ）. Condusion The serum IL-17 and slCAM-1 levels increase in patients with COPD during the acute exacerbation and stable periods, which indicates that IL-17 and sICAM-1 play a role in the course of inflammatory reaction of air passage of COPD.