直肠癌中VEGF-D和uPA的表达与侵袭转移的关系

Expression of vascular endothelial growth factor D （ VEGF - D） and urokinase - type plasminogen activator in human rectal carcinoma and their relationship with tumor invasion and metastasis

目的 探讨血管内皮生长因子-D（VEGF-D）和尿激酶型纤维蛋白酶原激活剂（uPA）在直肠癌中的表达特点及临床意义。方法分别应用免疫组化SP法、Westernblot法检测30例直肠癌组织及相应正常组织的VEGF-D和uPA的表达特点，分析其与临床病理分期的关系。结果VEGF-D和uPA在肿瘤组织和正常组织中均有表达，主要表达于胞浆，胞核也有。在肿瘤组织中的表达明显高于正常组织（P〈0．01）。VEGF-D或uPA的表达活性在DukesC＋D期明显高于A＋B期（P〈0．01），在有淋巴结转移组和远处转移组的表达高于相应无转移组（P〈0．05、P〈0．01），随着肿瘤组织分化程度的增高活性依次降低（P〈0．05）。结论VEGF-D和uPA在肿瘤组织中的高表达可能在肿瘤的发生发展过程中扮演重要角色。

Objective To explore the expression of endothelial growth factor-D （VEGF -D） and uroki- nase-type plasminogen activator（uPA） in rectal carcinoma and to reveal their correlation to the tumor invasion and metastasis. Methods The expression of VEGF - D and uPA in 30 cases with rectal carcinoma and normal tissue was detected by immunohistochemistry and Western blot. Results The expression of VEGF - D and uPA was de- tected both in tumor tissue and normal tissue,but significantly higher in tumor tissue（ P 〈 0. 01 ） , they expressed mostly in endoehylema. The expression of VEGF - D or uPA was significantly higher in Dukes＇ stage C ＋ D than that in Dukes＇ stage A ＋ B（P 〈0.01 ） , was also higher in tissues with lymph node metastasis or distant metasta- sis than those without metastasis（P 〈0.05,P 〈 0.01 ）. They were found to be lower in cancer tissue along with the differentiation degree increase （ P 〈 0. 05 ） . Conclusions The overexpression of VEGF - D and uPA in rectal carcinoma may play an important part in the tumorigenesis and progression of rectal carcinoma.