等电聚焦电泳法测定甘蓝自交不亲和性

Identification of the Self-incompatibility of Cabbage by Isoelectric Focusing

采用等电聚焦电泳法对１０个甘蓝自交不亲和系、５个亲和系的柱头、花粉蛋白质谱带进行了分析。对比自交不亲和系开花前２～３ｄ的柱头与成熟柱头，发现各试材在等电点为８．３～８．５的区域内有一条特异带，该带在成熟柱头中清晰可见，在开花前２～３ｄ的柱头中没有或较弱。据此特征推断该带即为Ｓ蛋白质特征谱带。以双蒸水和巯基乙醇作为提取液处理花粉，均未发现自交不亲和系共有的特征带。

Analysis of polyacy lamide gel isoelectric focusing was used in study of the proteins in stigmas and pollens of ten self-incompatible and five self-compatible lines of cabbage. By comparing the band patterns of mature stigmas and the stigmas of 2～3 days before blooming of self-incom patible lines, we found a specific band in PI 8.3～8.5 region. This band was clearly observed in mature stigmas, but not existed in young stigmas. It was S-locus specific glycoprotein band according to its characteristics. When the proteins of pollens were treated with ultrapure water and mercaptoethanol, no specific band was observed among the self-incompatible lines and the compatible lines.