胃良性和恶性病变组织中β-半乳糖凝集素3和接骨木凝集素的表达及其临床病理意义

Expression of galectin 3 and sambucus nigra agglutinin and their clinicopathological significance in benign and malignant lesions of stomach

目的研究胃良性和恶性病变组织中β-半乳糖凝集素-3（gal-3）和接骨木凝集素（SNA）的表达水平及其临床病理意义。方法将49例胃癌、20例癌旁组织、36例淋巴结转移灶和80例不同类型胃良性病变手术切除或胃镜活检标本常规制作石蜡包埋切片，gal-3染色方法为EnVision^TM免疫组织化学（免疫组化）法，SNA染色方法为ABC亲和组化法。结果胃癌组织gal-3和SNA表达阳性率明显高于癌旁组织及不同类型胃良性病变者（P〈0.05，P〈0.01）.阳性表达的胃良性病变标本和癌旁组织均呈不同程度不典型增生；淋巴结转移灶gal-3和SNA阳性率分别为63.9％和55.6％，与其相应的原发病灶gal-3和SNA阳性率（72.2％和66.7％）比较，差异无统计学意义（P〉0.05）。组织学分级Ⅱ级、浸润深度T1和T2及无区域淋巴结转移的胃癌组织gal-3和SNA表达阳性率明显低于组织学分级Ⅲ、Ⅳ级和浸润深度T3、T4及区域淋巴结转移者（P〈0.05）；淋巴结转移N1及无远处器官转移胃癌组织gal-3和SNA表达阳性率低于淋巴结转移N2、N3，站及远处器官转移的胃癌组织。但差异均无统计学意义（P〉0.05）；胃癌组织中，31例gal-3表达阳性病例中SNA阳性22例，两者表达呈明显一致性（X^2=6.59，P〈0.05）。结论gal-3和SNA表达水平可能是反映胃癌发生、进展及其临床生物学行为的重要凝集素分子标记物。

Objective To study the expressive levels of galectin-3（gal-3） and sambucus nigra agglutinin （SNA） and their clinicopathological significance in the benign and malignant lesions of stomach. Methods EnVison^TM immunohistochemistry for assaying gal-3 expressive level and ABC cytochemistry for determining SNA expressive level were used in conventional paraffin-embedded sections from specimens of gastric cancer（n=49）, peritumoral tissues（n=20）, metastastic loci of lymph nodes（n=36）, and different types of benign lesions（n=80）. Results The positive rates of gal-3 and SNA were significantly higher in gastric cancer tissues than those in peritumoral tissues and different types of benign lesions （P〈0.05, P〈0.01）. The positive cases of gal-3 and/or SNA in peritumoral tissues and benign lesions showed mild-to severe-atypical hyperplasia of mucous epithelial cells. No difference was found between the primary loci and metastatic foci in gal-3 and SNA expressions（P〉0.05）. The positive rates of gal-3 and SNA were significantly lower in histologic grade Ⅱ, infitrating depth T1, T2 and no-metastasis of regional lymph node than those in histologic grade Ⅲ, Ⅳ, infitrating depth T3,T4 and metastasis of lymph node in gastric cancer（P〈0.05）. The positive rates of gal-3 and SNA were higher in lymphnode metastatic site Ni and no-metastasis of distant organs than those in lymphnode metastatic site N2, N3 and metastasis of distant organs, but no significant difference was found （P〉0.05）. The consistence was found between the expression of gal-3 and SNA in gastric cancer tissues（X^2=6.59,P〈0.05）. Conclusions The expressive levels of gal-3 and SNA may be important molecular markers of lectins for reflecting the carcinogenesis, progression and biological behaviors in gastric cancer.