镉对中国林蛙蝌蚪肝内FOS蛋白和金属硫蛋白的影响

Effect of Cadmium on Fos and Metallothionein Expression in Liver Cells in of Rana chensinensis Tadpole

将生长至26～27期中国林蛙（Rana chensinensis）蝌蚪暴露于0．05、0．1、0．2和0．dmg／L的Cd2＋水体中至完全变态，用免疫细胞化学方法检测变态后亚成体肝细胞的原癌基因FOS蛋白和金属硫蛋白（MT）。结果显示，FOS和MT在Cd2＋处理组肝细胞中的阳性反应强度及其定位具有一致性。0．05mg／L组肝细胞中FOS和MT为弱阳性反应，与对照组相比差异不显著（P〉0．05）；0．1和0．2mg／L组的阳性表达差异显著（P〈0．05）；0．4mg／L组阳性表达差异极显著（P〈0．01）。可见在0．05—0．dmg／L的Cd2＋范围内对肝细胞中FOS和MT的影响随着Cd2＋的浓度增加而逐渐增强。

Rana chensinensis tadpoles at the stage 26 - 27 was placed in tap water with 0.05, 0.1, 0.2, 0.4 mg/ L Cd2＋ until heteromorphy. Localizations of FOS and metallothionein （MT） on liver cells in Rana chensinensis subadult were investigated using immunocytochemistry. The results showed that FOS and MT were positive co-ex- pressed on liver cells in Cd2＋ treated groups. In Contrast to controlled group, FOS and MT were weak positive expressions, the difference is not obvious（ P 〉 0.05 ） , in 0.1 and 0.2 mg/L Cd2＋ groups, the difference of positive expressions was obvious（P 〈 0.05 ）, the staining intensity was significantly higher in 0.4 mg/L Cd2＋ group （P 〈 0. 01 ）. Among the 0.05 -0.4 mg/L Cd2＋ concentration, the effect of Cd2＋ on FOS and MT was increasing with concentration up.