摘要
以谷氨酸棒杆菌FC22(Phe-+Tyr-+5MTr)为出发菌株,经过紫外线诱变,定向选育出具有磺胺胍抗性目的突变株FC523(Phe-+Tyr-+5MTr+SGr).通过正交实验对FC523的发酵培养基进行优化,并通过单因素实验对温度、pH和转速等发酵条件进行研究.在最佳条件下,该菌株摇瓶积累L-色氨酸最高可达8.6 g/L.
A sulfonamide resistant mutant FC523 （Phe-＋Tyr-＋5MTr-SGr） was obtained from Corynebacterium glutarnicurn FC22 （Phe- ＋Tyr- ＋SMTr） by UV treatment. The culture medium was optimized by orthogonal design experiment and fermentation conditions were studied by single factor experiment. Tryptophan was accumulated to 8.6 g/L by strain FC523 at the optimized conditions.
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第3期84-88,共5页
Journal of Fujian Normal University：Natural Science Edition
关键词
L-色氨酸
诱变育种
磺胺胍
抗性筛选
发酵
L-tryptophan
mutation breeding
sulfonamide
resistance screen
fermentation
