大熊猫种群数量调查方法的新突破

A NEW BREAK THROUGH ON THE NUMBER INVESTIGATION METHOD OF THE GIANT PANDA′S POPULATION

作者应用寡核苷酸基因ＬＺＦ－Ｉ和Ｆ２ＺＧＰ９６０６０８０１探针，对野生大熊猫粪便样品材料作ＤＮＡ指纹图分析，确认大熊猫野生种群的个体数量和家系，获得了满意的结果。文章还介绍了在不同自然保护区、山系或行政区域内，大面积上作大熊猫的种群数量调查采用的方法：Ａ在已知大约数量时，则以１∶５采集粪便样品为宜；Ｂ在未知数量及密度时，则以面积和路线法布点，采集粪样，避免遗漏，影响真实的种群个体确切数量；Ｃ利用空白地形图标注粪样编号，用以核对大熊猫的数量、家系、领域以及每只个体的性别与大致年龄结构，便于评估大熊猫的数量、分布、栖息地的质量与发展趋势，有利于制定保护管理规划，更好地保护大熊猫物种。本法突破了现有陆生哺乳动物数量调查方法，并具有推广和应用价值。

Using the oligonucleotide probes of LZF-I and F2ZGP 96060801 and the materials of excrements of giant pandas,we have obtained an ideal result on determining the numbers and families of wild giant pandas,and here we will report the methods of determining the number,sexual distinction,family and the structure of age of wild giant panda′s population by means of DNA fingerprinting and PCR and extracting DNA from the excrements of wild giant pandas,and we hope it can solve the problems of determining giant panda′s number of Nature Reserve,the distribution of individuals,the quality of Nature Reserve and the developing trend of Nature Reserve.