脂联素对大鼠血管平滑肌细胞VCAM-1和ICAM-1表达的影响

Effect of adiponectin on expression of VCAM-1 and ICAM-1 in rat vascular smooth muscle cells

目的探讨脂联素对肿瘤坏死因子α(TNF-α)诱导大鼠主动脉血管平滑肌细胞血管细胞黏附分子1(VCAM-1)和细胞间黏附分子1(ICAM-1)的表达及其意义。方法复苏培养大鼠血管平滑肌细胞,通过逆转录聚合酶链式反应(RT-PCR)和免疫蛋白印迹法(Western blot)观察脂联素对TNF-α诱导的血管平滑肌细胞VCAM-1、ICAM-1蛋白及mRNA的影响。结果TNFα-(10 ng/ml)刺激后血管平滑肌细胞VCAM-1和ICAM-1表达明显增强(P<0.01);脂联素对其则呈浓度依赖性抑制作用。结论脂联素可抑制TNF-α诱导的血管平滑肌细胞VCAM-1和ICAM-1的表达,这可能对减轻血管壁的炎症反应、延缓动脉粥样硬化的发生、发展起一定作用。

Objective To investigate the effects of adiponeetin on the expression of vascular cell adhesion molecule-1 （VCAM-1） and intercellular adhesion molecule-1 （ ICAM-1 ） induced by tumor necrosis factor-alpha（TNF-α） in rat vascular smooth muscle cells （VSMCs）. Methods Incubated with 10 ng/ml of TNF-α, the expressions of VCAM1 and ICAM-1 in VSMC were detected by reverse transcription polymerase chain reaction （RT-PCR） and Western blot analysis respectively. Results The expressions of VCAM-1 and ICAM-1 were markedly increased in VSMCs after treated with 10 ng/ml of TNF-α compared to those before （P〈0.01）, which was inhibited by adiponectin in a dosedependent manner. Conclusion Adiponeetin may inhibit TNF-α-induced expressions of VCAM-1 and ICAM-1 in VSMCs, which may be helpful in reducing vascular imflammatory response and antiatherosclerosis.