摘要
采用土壤法培养和组织培养的方法,分析小叶海金沙配子体的发育和快速繁殖技术。研究发现小叶海金沙的孢子呈金黄色,极面观钝三角形,赤道观为半圆形或圆形,三裂缝。孢子萌发类型为书带蕨型,原叶体发育类型为铁线蕨型。在1/4MS培养基上,其增殖率最高。
Gametophytic development and rapid-proliferation techniques of Lygodium scandens （Linn.） Sw. were studied. The process of spore germination and gametophytie development were observed and recorded. The spores were gold, blunt triangular polar view, the equator concept as a semicircular or circular and three cracks. The pattern of spore germination was the Anemiatype and the gametophyte development was the Adiantum-type. Lygodium scandens（Linn.） Sw. was cultured with tissue culture for rapid propagation, and the optimal medium for prothallium grows and young sporophyte multiplication was 1/4 MS medium.
出处
《湖北农业科学》
北大核心
2009年第4期907-909,共3页
Hubei Agricultural Sciences
基金
广西自然科学基金项目(桂科自0728095)
关键词
小叶海金沙
孢子萌发
配子体发育
快速繁殖
Lygodium scandens （Linn.） Sw.
spore germination
gametophyte development
rapid propagation
