摘要
本文建立的猪血浆转铁蛋白双抗夹心ELISA灵敏度为61ng/ml.批内和批间变异系数为7.84％和8.85％,测定112例各日龄健康猪血浆转铁蛋白水平为2687.27-3173.15μg/ml。与火箭免疫电泳所测结果的相关系数达0.9586,尤其适合于大批量样品的快速测定.
Sandwich ELISA of transferrin in pig plasma was established. The sensitivity was 61ng/ml and the variation coefficient (VC) between and within batches were 7.84% and 8.85% respectively. The average transferrin levels of 112 plasma samples from healthy pigs at various age ranged from 2687.27 to 3173.15μg/ml. The correlation coefficient between rocket imraunoelectrophoresis assay and sandwich ELISA was 0.9586. This method is especially applicable to quick measurement for great batch of samples.
出处
《江苏农学院学报》
CSCD
1990年第1期67-70,共4页
Jiangsu Agricultural Research
