摘要
从10株抗鸡新城疫病毒(NDV)特异性单克隆抗体(MAbs) 中筛选出2株MAbs—M22和F23,分别用作包被抗体和酶标抗体而建立的MAbs—夹心ELISA试验,对提纯NDV的最小检出量为25ng/ml病毒蛋白.NDV鸡阳性血清对本试验的特异性阻断作用和与其他禽类病毒交叉反应的阴性结果,证明本试验对NDV的特异性。从临床疑为鸡新城疫的724只病鸡的口腔棉试样品中共检出阳性502只,其中200个样品与病毒分离试验结果完全一致,两者均查出阳性鸡176只。
A monoclonal antibody based sandwich ELISA for the detection of NDV antigen was developed. Two MAbs, M22 and F23 were sclected from 10 MAbs with specificity to NDV and used as coating antibody and HRPO conjugated antibody respectively. The lower limit of this assay as determined by the use of purified NDV was 25 ng viral protein/ml. The specificity of the assay for NDV was further verined by the blocking activity of chicken anti-NDV serum and the negative reaction with pathogens other than NDV. 502 out of 724 oral swab samples from field cases suspected with ND were tested as positive by this as- say. For comparison, virus detection of 200 out of 724 samples were conducted simultaneously by MAb-based ELISA and virus isolation, 176 positives were detected by both.
出处
《江苏农学院学报》
CSCD
1990年第2期41-45,共5页
Jiangsu Agricultural Research
关键词
鸡新城疫病毒
抗体
检测
newcastle disease virus (NDV)
monoclonal antibody
sandwich ELISA assay
