摘要
采用测定光合放氧速率和荧光动力学的方法,研究分析了光照强度、温度和pH对雨生红球藻Haematococcus pluvialis CG-11绿色游动细胞阶段光合作用特性的影响。结果表明,H.pluvialis CG-11光合作用的饱和光强为109.1μmol/m2·s,最大光合放氧速率为75.9μmolO2/mg Chla·h;适宜的生长温度范围在25—30℃之间,温度在25℃时光合速率最大;pH在7.5—8.0范围内,光合效率较高,在pH为7.5时放氧速率最大,为75.5μmolO2/mg Chla·h。在实验pH条件下,H.pluvialisCG-11叶绿素荧光动力学参数呈现出相似的趋势,在6.0—7.5范围内,Fv/Fm、Fv/F0、ΦPSⅡ和ETR值随pH升高而增大,pH为7.5时达到最大值,pH超过7.5时,Fv/Fm和Fv/F0值明显下降,而ΦPSⅡ和ETR的下降趋势不明显。
The effects of light intensity, temperature and pH on photosynthetic characters of Haematococcus pluvialis CG-11 were studied by measuring photosynthetic 02 evolution and fluorescence kinetics. The results showed that the saturation light intensity was 109.1 μ,ol/m^2· s of H. pluvialis CG-11, with the maximum photosynthetic oxygen evolution rate of 75.9 μmol O^2/mg Chlα· h; the range of suitable growth temperature was between 25℃ to 30℃ , the maximum photosynthetic rate was obtained at 25 ℃ ; the photosynthetic efficiency kept quite high at the pH range of 7.5--8.0,with the maximum photosynthetic oxygen evolution rate of 75.5 μmol O2/mg Chla ·h at pH value of 7.5. Under experimental conditions of pH, a similar tendency manifested in the chlorophyll fluorescence kinetics parameters for H. pluvialis, at the pH range of 7.5--8.0, the Fv/Fm , Fv/F0 , ФPS Ⅱ and ETR increased following the rising of pH, with their maximum values at pH 7.5, Fv/ Fm and Fv/F0 decreased significantly over pH 7.5, while the decreasing trend didn' t change obviously in ФPS Ⅱ and ETR.
出处
《水生生物学报》
CAS
CSCD
北大核心
2009年第3期400-405,共6页
Acta Hydrobiologica Sinica
基金
珠海市科技重大项目(PB20041018)资助
关键词
雨生红球藻
光照强度
温度
PH
叶绿素荧光
光合放氧速率
Haematococcus pluvialis
Lighting intensity
Temperature
pH
Chlorophyll fluorescence
photosynthetic oxygen evolution rate