过氧化氢处理时间对异种骨移植替代物的影响

INFLUENCE OF IMMERSED TIME BY HYDROGEN DIOXIDE ON XENOGENEIC BONE SUBSTITUTE

目的探讨H2O2处理时间对牛松质骨粒生物学特性的影响。方法健康24月龄秦川牛10头,雌雄不限,体重150～170kg。取牛肱骨头松质骨,切割成5mm×5mm×5mm大小骨粒,分别以8.8mol/LH2O2浸泡0、12、24、36、48、60、72h后,行灰化组分测定、扫描电镜观察、X射线能谱分析及显微CT观察,研究牛松质骨粒的组分、结构、骨质及骨量变化。结果随H2O2处理时间延长,骨粒有机物含量逐渐减少,0～24h和60～72h有机物含量差异有统计学意义(P<0.05);24～60h无明显变化(P>0.05)。骨粒中钙、磷元素含量逐渐减少,60h后几乎检测不到(P<0.05)。骨密度、骨矿物质含量呈下降趋势,且60h后下降明显(P<0.05);骨小梁逐渐变细,骨小梁分离度增加。结论H2O2能有效去除异种骨粒中有机物抗原成分,但处理时间超过60h会严重破坏骨粒组分和结构,故合理掌握处理时间对消除异种骨移植替代物抗原性并维持其生物学特性具有重要意义。

Objective To study the in uence of the immersed time by hydrogen dioxide on the characteristics of bovine cancellous bone granules in various periods.Methods Ten 24-month-old Qinchuan bovine, male or female, weighing 150-170 g, were selected.Cancellous bone granules from metaphysic of bovine long bone were cut into cubes of 5 mm × 5 mm × 5 mm and immersed by 8.8 mol/L hydrogen dioxide for 0, 12, 24, 36, 48, 60 and 72 hours separately.Determination of ash, scanning electron microscope, X-ray energy spectrum and micro CT were used to investigate the changes of composition, structure and quality of bone.Results With the immersed time increasing, the contents of organics in the bone cancellous were reduced gradually, and obviously decreased during the periods of 0 to 24 hours and 60 to 72 hours（P 〈 0.05）.The contents of calcium and phosphorus decreased gradually, they could not be detected almost after 60 days（P 〈 0.05）.Bone mineral density and bone mineral content were decreased obviously after 60 hours（P 〈 0.05）.The bone trabecula became slimmer and trabecular spacing became larger.Conclusion Hydrogen dioxide can be used to remove the antigen in xenogeneic bone;however as the time increasing（more than 60 hours） the composition and structure will be damaged.Thus it is important to control the immersed time for maintaining the biological characteristics of xenogeneic bone substitute as well as eliminating antigen by hydrogen dioxide.