肝内转移和多中心发生的多结节肝癌蛋白质表达谱的差异分析

Protein profiles of multinodular hepatoceHular carcinoma with multicentric occurrence or with intrahepatic metastasis

目的比较分析多结节肝癌的肝内转移癌灶和多中心发生癌灶的蛋白质表达谱，为更加准确的分子分型方法提供实验依据。方法对5例肝内转移（IM）和6例多中心发生（MO）的多结节肝癌按照结节大小分为IM1、IM2、MO1、MO2四组，联合双向凝胶电泳与质谱技术分析多结节肝癌的蛋白质表达谱；并用Western blot验证质谱结果。结果双向凝胶电泳联合质谱技术鉴定IM1、IM2、MO1、MO2四组共30个差异蛋白质点，确认为25种差异表达的蛋白质；GeneOntology分类显示其与细胞运动、信号转导、氧化还原、脂类代谢、氨基酸代谢等有关。结论多结节肝癌肝内转移和多中心发生的蛋白质表达不同；双向凝胶电泳联合质谱技术可作为二者分子分型的方法，有利于临床医师对肝癌患者实施治疗和判断预后。

Objective To analyze the protein expression profiles of multinodular hepatocellular carcinoma （HCC） with multicentric occurrence （MO） or with intrahepatic metastasis （IM）. Methods 5 IM and 6 MO patients were divided into groups of IM1, IM2, MO1 and MO2 according to the size of node of HCC. Two dimensional gel electrophoresis （2-DE） and mass spectrum were Used to analyze the protein expression profiles. Western blot was used to confirm the results obtained by mass spectrum. Results 2-DE of IM1, IM2, MO1 and MO2 indicated that 30 protein dots were differentially expressed in these tumors. By mass spectrum, 25 proteins were identified. Gene ontology classification indicated that these proteins are associated to cell movement, signal transduction, oxidoreduction, lipid metabolism, and amino acid metabolism. Conclusion The protein expression profiles of IM is different from that of MO, 2-DE and mass spectrum can be used to identify the molecular markers of IM and MO of HCC.