摘要
2001年从福建某猪场分离到1株H5N1亚型猪流感病毒(SIV)A/Swine/Fujian/1/01(SW/FJ/1/01)。SW/FJ/1/01对小鼠具有强致病性,致死率为100%,为进一步研究SW/FJ/1/01对BALB/c小鼠致病性的分子基础,本研究构建了SW/FJ/1/01的8个节段重组质粒构成的反向基因操作系统,成功拯救了病毒(R-SW/FJ/1/01)。R-SW/FJ/1/01和野生型SW/FJ/1/01对BALB/c小鼠致病性没有差别。SW/FJ/1/01反向基因操作系统的建立为进一步阐明H5N1亚型SIV对哺乳动物模型BALB/c小鼠的致病机制等方面的研究奠定了基础。
An infectious clone of H5N1 swine influenza virus A/Swine/FuJian/1/01 was generated using an eight-plasmid reverse genetics system. The plasmids transfected into cells and virus （R-SW/FJ/1/01） rescued showed the same biological properties as the wild virus （W-SW/FJ/1/01）. Both viruses could be recovered from the lung of the BALB/c mice 3 days after inoculated intranasally with 106 EIDs0 dose. The successful establishment of the reverse genetics system of SW/FJ/1/01 will play an important role in the studies of the molecular basis of the evolution and the relationship between the structure and function of H5N1 subtype swine influenza viruses.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2009年第5期400-402,共3页
Chinese Journal of Preventive Veterinary Medicine
基金
国家自然科学基金项目(C020306-30440008)
