新疆维吾尔族人2型糖尿病患者AMPKα2基因多态性的分析研究

The Genetic Polymorphism analysis of AMPKα2 of the type 2 diabetes in Xinjiang Uygur Population

目的:研究腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)单核苷酸多态性(SNPs)rs2143754位点与新疆地区维吾尔族人2型糖尿病之间的关系。方法:以聚合酶链式反应-限制性内切酶长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)技术,对150例2型糖尿病和150例正常对照者AMPK-rs2143754位点进行基因分型。结果:AMPKα2位点T/T、G/T和G/G基因型频率在病例组为(40%,51%和9%),正常对照组为(28%,49%和29%),两组基因型和等位基因频率分布差异有显著性(P<0.05)。结论:AMPKα2多态性与新疆维吾尔族人2型糖尿病有明显相关性。

Objective： To investigate the correlation between single nucleotide polymorphisms （SNPs） rs2143754 of AMPK gene and type 2 diabetes in Xinjiang Uigur population. Method： A 1：1 Matched Case-control Study was conducted with 150 cases of type2 diabetes and 150 population-based controls. AMPKα2 gene polymorphisms genotypes were detected by PCR-RFLP. Results： The distribution ofT/T, G/T and G/G genotype of the AMPK-rs2143754 were （40%, 51% and 9%） in type2 diabetes patients, whereas （28%, 49% and 29%）in the controls. The frequencies of alleles and genotypes of AMPKa2 in type2 diabetes patients were significantly different from those of the controls （P〈0.05）. Conclusion： The results suggested that the AMPKα2 were correlated with type2 diabetes in Xinjiang Uigur Population.