郏县红牛POU1F1基因第6外显子HinfⅠ、AluⅠ和PstⅠ位点遗传变异及其与生长发育的关系

Genetic variations of HinfⅠ,AluⅠ and PstⅠ loci within POU1F1 gene exon 6 and its association with growth traits in Jiaxian Red Cattle

【目的】研究POU1F1基因第6外显子HinfⅠ、AluⅠ和PstⅠ酶切位点多态性对郏县红牛生长发育性状的遗传效应。【方法】以144头郏县红牛为研究材料,用PCR-RFLP方法检测POU1F1基因第6外显子HinfⅠ、AluⅠ和PstⅠ基因座多态性,并分析其基因型间遗传变异与生长发育的关系。【结果】451bp的POU1F1基因第6外显子片段被HinfⅠ酶切后表现多态;AA、AB和BB基因型频率分别为0.1875,0.3056和0.5069,A和B等位基因频率分别为0.3403和0.6597,处于Hardy-Weinberg不平衡状态;该位点纯合度为0.5510,杂合度为0.4490,有效等位基因数为1.8148,多态信息含量为0.3482,属中度多态,可作为该群体遗传资源评价的建议性指标。而POU1F1基因第6外显子AluⅠ和PstⅠ基因座均未检测到多态性,仅发现AA基因型,表明这些基因座比较保守。HinfⅠ多态性与郏县红牛生长发育性状的相关分析表明,不同基因型与郏县红牛体高、腰角宽、胸围、尻长、十字部高、管围、体质量等指标均无显著相关性(P>0.05);BB基因型个体体长显著高于AB和AA基因型个体(P<0.05),提示B等位基因可能对体长有显著影响。【结论】BB基因型可作为郏县红牛体长性状标记辅助选择的DNA标记。

[Objective] The objective of the present study was to study the genetic effects of polymorphisms of Hinf Ⅰ ,Alu Ⅰ and Pst Ⅰ loci within POU1F1 gene exon 6 on Jianxian Red cattle growth traits. [Method] POU1F1 gene exon 6 polymorphic fragments were detected by PCR-RFLP technique using 144 Jianxian Red cattle. Association analysis of POU1F1 gene exon 6 polymorphism with growth traits was also conducted. [Result] The results showed that 451 bp PCR products digested with Hinf Ⅰ demonstrated polymorphisms. Moreover, the frequencies of allele AA, AB and BB genotype of Jiaxian red cattle were 0. 187 5,0. 305 6 and0. 506 9,accordingly,the frequencies of allele A and B were 0. 340 3 and 0. 659 7 respectively. The homozygositiy,heterozytosiy, the effective allele numbers,and PIC value of POU1F1 gene exon 6 HinfⅠ locus were 0. 551 0,0. 449 0,1. 814 8 and 0. 348 2 respectively. As this locus belonged to the middle polymorphic index,it was suggested to be an effective evaluation parameter for studying genetic resource. However, no polymorphism was found at POU1F1 gene exon 6 Alu Ⅰ and Pst Ⅰ loci. The correlation of Hinf Ⅰ polymorphisms of POU1F1 gene exon 6 and growth traits was analyzed and the result showed that there was significant relationship between Hinf Ⅰ polymorphism and body length （P〈0.05）. The individual with genotype BB had longer body length than the individual with genotype AB and AA, suggesting the allele B had significant effect on body length of Jianxian red cattle. [Conclusion] The genotype BB was significantly correlated with the fine performance and the selection of the allele B was better for the improvement.