小麦大穗材料西农9814外源物质的分子细胞遗传学检测

Molecular cytogenetics identification of alien chromatin in Xinong 9814

【目的】检测小麦品种西农9814的外源物质,分析其遗传基础,为西农9814的进一步改良及应用提供依据。【方法】以中国春为对照,以西农9814及其亲本临旱957和西农1718为供试材料,采用基因组原位杂交(GISH)、SCAR标记和微卫星(SSR)技术进行外源物质检测。【结果】通过GISH分析,推测西农9814是1BL-1RS或6BL-1RS的小麦-黑麦易位系;SCAR分析检测发现,西农9814和其亲本西农1718均含有黑麦1.5kb的1RS特征条带,通过SSR分析发现,在黑麦和西农9814中,1BS上的2对引物未扩增出1BS条带,而6BS上的2条引物扩增出了6BS条带,证实西农9814为黑麦的1BL/1RS易位系,而且为1RS的整臂易位。【结论】小麦品种西农9814为黑麦的1BL/1RS易位系。

[Objective] The research was conducted to detect xogenous hereditary property of Xinong 9814. [Method] The detection of rye chromatin was done by means of genomic in situ hybridization （GISH）,sequence characterized amplified region （SCAR） and microsatellites（SSR）. [Result] Genome in Situ Hybridization （GISH） indicated that Xinong 9814 was 1BL/1RS or 6BL/1RS,because the traslocation chromosomes had satellites. SCAR displayed a single 1.5 kb rye band which could be amplified in Xinong 9814 and its mother Xinong 1718. SSR analysis displayed the two primers in 1BS could not amplify bands, but 6BS could amplify bands, which proved Xinong 9814 was 1BL/1RS. [Conclusion] Xinong 9814 was 1BL/1RS traslocation line.