七叶树溃疡病病原真菌的分离与鉴别

Isolation and identification of two kinds of canker pathogen of Aesculus chinensis

【目的】分离和鉴别引起七叶树溃疡病的病原菌,为制定合理的防治技术提供依据。【方法】采用组织分离培养、人工接种、形态观察及核糖体转录间隔区序列(ITS-rDNA)分析,对引起七叶树溃疡病的水渍型溃疡病病原菌NW397和干腐型溃疡病病原菌NW3362种真菌进行鉴别与比较。【结果】引起七叶树溃疡病的病原为2种不同的真菌,并表现出不同的危害特征。水渍型溃疡病菌NW397 ITS-rDNA序列与多主葡萄座腔菌(Botryosphaeria dothidea Sacc.)等的序列同源性高达99%;干腐型溃疡病菌NW336ITS-rDNA序列与乌饭树拟茎点菌(Phomopsisvaccinii)等的同源性高达95%以上。【结论】七叶树水渍型溃疡病菌定名为多主葡萄座腔菌(Botryosphaeria do-thidea Sacc.),干腐型溃疡病菌定名为梨干枯拟茎点菌(Phompsis fukushii Tanaka et Mill)。

[Objective] The research studied the isolation and identification of the canker pathogen occurred on Aesculus chinensis Beg. [Method] Traditional Kock＇ s technology and ITS sequence analysis technology were used to ideatify and compare two canker pathogens： NW397 and NW336. [Result] Two kinds of canker pathogen of Aesculus chinensis were identified. Pathogen of immerse canker had over 99% homogeneous with fungi Botryosphaeria dothidea Sacc. Pathogen of dry rot canker had over 95 % homogeneous with fungi Phornopsis vaccinii, and so on. [Conclusion] The pathogen of immerse canker （isolate NW397） was identified as Botryosphaeria dothidea Sacc ,and the dry rot pathogen （isolate NW336） was identified as Phornopsis fukushii Tanaka et Mill. They were two different pathogen fungi with different disease symptoms.