摘要
介绍一种用四氦唑蓝为底物的酶反应定量比色分析法。方法用ＭＴＴ法测定了丙烯酰胺对体外培养的大鼠胚胎中脑神经细胞的毒性。结论ＭＴＴ法用于测定细胞毒性作用具有快速，正确等优点。
Objective To introduce a quantitative colorimetric assay based on a tetrazolium salt (MTT). Methods To testify the toxic effect of acrylamide on neurons from rat embryo midbrain in vitro by using MTT. Results The absorbance was directly proportional to the number of cells and the linearity was good in the range from 1×10 4 to 4×10 5 cells/ml culture fluid;Acrylamide was proved to be a cytotoxicant for neuron and its IC 50 value was 85 ug/mg.Conclusion The method of MTT can be used to measure neuron cytotoxicity.The main advantages of this assay are its rapidity and precision.
基金
国家自然科学基金
