前庭康复过程中Ⅰ组代谢性谷氨酸受体亚型mGluR5在前庭内侧核中的变化

Expression change of mGluR5 in rat MVN after unilateral labyrinthectomy

目的:观察单侧迷路破坏术后前庭内侧核(MVN)内Ⅰ组代谢性谷氨酸受体亚型mGluR5的表达变化。方法:成年雄性Wistar大鼠30只,分为迷路破坏组(24只)和对照组(6只),前者破坏单侧迷路,对照组手术方式相同但保持迷路完好。单侧迷路破坏术后,通过免疫组织化学、原位杂交组织化学法检测不同存活时间(术后12h、36h、7d)2组动物MVN内mGluR5的表达变化。结果:单侧迷路破坏术后可诱导同侧MVN区Ⅰ组代谢性谷氨酸受体亚型mGluR5增高,术后12h最高,术后36h开始降低,至术后7d后和对照组差异无统计学意义;对侧和术侧的变化趋势相同。结论:单侧迷路破坏术后可诱导MVN区Ⅰ组代谢性谷氨酸受体亚型mGluR5增高。初级前庭传入或中枢前庭神经元的静息放电降低可能与Ⅰ组代谢性谷氨酸受体亚型mGluR5增高有关,但其在前庭代偿中的作用尚有待研究。

Objective：To observe the expression of mGluR5 in the medial vestibular nucleus（MVN） following unilateral labyrinthectomy（UL）. Method： Thirty Wistar rats were randomly derided into two groups. Twenty four animals recieced unitaleral labyrinthecomy while the others maintained labyrinthine well. After secting left labyrinthine, the change of mGluR5 was induced by immunohistochemistry, in situ hybridization. Result：mGluR5 was increased in lesioned side MVN after unilateral labyrinthectomy. The 12 h post-ULwas highst. Then it was decrease in 36 h post-UL,while 7 d posVUL was same as control group. The change of contralateral was same as that in ipsilateral. Conclusion：UL can induce increase of mGluR5 in the MVN. The reduced resting discharge in the primary vestibular afferents or in the central vestibular neurons may be responsible for the change of mGluR5. However the significance of the change of mGluR1 in the vestibular compensation is still unknown.