爬行卫矛贮藏蛋白的初步研究

Study of Vegetative Storage Protein in Euonymus fortunei var.Radicans

以不同季节的爬行卫矛枝条为研究对象,利用光学显微技术和SDS-PAGE技术来研究其休眠期和生长期枝条中贮藏蛋白的分布情况。经汞—溴酚蓝染色后的石蜡切片显微观察表明:休眠期枝条细胞的液泡中有大量蓝色小球状蛋白颗粒,有的甚至充满整个液泡,而在生长期枝条细胞中则未发现这种蛋白颗粒的存在。SDS-PAGE结果表明:与生长期枝条样品相比,有一种分子量约为45 kD蛋白质在休眠期枝条大量特异表达,这可能就是贮藏蛋白。

Vegetative storage proteins （VSPs）in the Euonymus fortunei var. Radicans were identified by using optical microscopy and SDS-PAGE. Under optical microscope, deep blue masses in a granular appearance were observed in the secondary xylem parenchyma cells of the dormancy season barks after stained with mercury-bromophenol blue. However, there was no granular protein in the development season barks. Compared with SDS-PAGE results of the development season barks , there was a 45 kD major protein expressed in the dormancy season barks, which was probably the vegetative storage protein of Euonymus fortunei var. Radieans.