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神经节苷脂GM1在BMSCs向神经细胞分化的作用

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摘要 目的研究体外使用神经节苷脂GM1诱导骨髓间充质干细胞(BMSCs)向神经细胞分化的作用。方法从健康志愿者髂骨处抽取骨髓5mL,体外分离、培养、扩增后,用含有10μg/mL、60μg/mL、90μg/mLGM1的无血清培养基诱导BMSCs向神经细胞方向分化。0.5h、3h、6h、24h后使用倒置显微镜和免疫细胞化学方法观察诱导前后细胞的变化。结果诱导6h后各组均有部分细胞胞体收缩、伸长突起,表现出神经细胞的形态。0.5h、3hNF、MAP-2阳性细胞比例无统计学意义(P>0.05),6h、24hNF、MAP-2阳性细胞比例无统计学意义(P>0.05),6h、24h分化率高于0.5h和3h(P<0.05),含60μg/mLGM1的无血清培养基诱导后细胞的分化率高于10μg/mL组和90μg/mL组。结论含60μg/mLGM1的无血清培养基可以有效地诱导BMSCs向神经细胞分化。
作者 赵永旺 李光来 薛芳 李东芳 连霞
机构地区 山西医科大学第二医院
出处 《中西医结合心脑血管病杂志》 2009年第5期538-539,共2页 Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
关键词 骨髓间充质干细胞 神经节苷脂GM1 细胞分化 神经细胞
分类号 R329 [医药卫生—人体解剖和组织胚胎学]
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参考文献13

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中西医结合心脑血管病杂志
2009年 第5期

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