新型两亲性超枝状大分子聚己内酯／聚缩水甘油醚纳米粒子实验研究

Study on nanopartide of amphiphilicpoly （epsilon-caprolactone）-b-hyperbranched polyglycidol

目的选择合适实验条件将两亲性超枝状大分子聚己内酯／聚缩水甘油醚制备成纳米粒子，以进一步应用于靶向药物传递载体。方法称取聚己内酯／聚缩水甘油醚（通过H^1NMR和GPC定性）30mg，溶于3mL丙酮和2mL乙醇混合溶剂中，在搅拌速度为250r／min的条件下。将上述溶液按250uL·min^-1速度注入30mL的蒸馏水中，搅拌过夜，减压挥发除掉溶剂，冷冻干燥得到纳米粒子。结果通过透射电镜观察，所得纳米粒子为球形，形状均匀，粒径约在50—100nm之间。结论本研究通过实验，首先合成分子量2×10^4U左右的聚己内酯／聚缩水甘油醚聚合物，通过×选择合适的溶剂。得到聚己内酯／聚缩水甘油醚纳米粒子。该方法操作简易，环境友好，易于后处理，所得纳米粒子大小均匀，通过后修饰，这种纳米粒子将有望被应用于体内靶向传递。

Objective Nanoaprticles of amphiphilic poly （epsilon-caprolactone）-b-hyperbranehed polygly cidol（PCL-b-HPG） were synthesized for further application as targeted drug delivery vehicles. Methods 30 mg PCL-b-HPG that was characterized by H^1NMR, GPC was dissolved in the blend solution of 3 mL of acetone and 2 mL of ethanol. This solution was added dropwise to 30 mL of distilled water at the rate of 250uL. rain-1 and magnetically stirred overnight at 250 rpm. After completion of solvent evaporation the nanoparticles （NPs） were collected by centrifugation, washed and dried. Results The size and morphology of NPs were characterized by TEM.The NPs were spherical in uniform shape with diameter ranging from 50 to 100 nm. Conclusion In present study, nano-scaled amphiphilie PCL-b-HPG were synthesized. The procedure which employed appropriate solvent was proved to be efficient, environmental friendly and easy post-processing. By further modification, the resulting nanomaterials with uniform scale offered opportunities for developing better targeting delivery system in vivo.